Protein Sciences & Analytics

High-quality protein production, analytical assays, and developability insights

Mosaic produces and qualifies proteins and analytical materials and delivers robust binding assays to support discovery, lead triage, and candidate selection. Our protein sciences and analytics teams work in close integration to ensure assays are technically sound, reproducible, and decision-relevant. Analytical characterization complements cell-based assays by confirming stability, aggregation, and developability of biologic candidates.

We support discovery programs with protein production, assay development, and comprehensive biophysical and biochemical characterization to assess quality, stability, and developability early.

Binding Assays

Label-free, real-time characterization of protein–protein interactions using purified or non-purified samples on industry-leading platforms.

SPR and BLI kinetics (KD, ka, kd)

Epitope binning and competition studies

Solution-based orthogonal confirmations

Species cross-reactivity profiling

Affinity ranking and concentration determination

Protein Sciences

Expert execution of protein production and modification to enable discovery and characterization workflows.

Platform-agnostic expression and purification (bacterial, cell-free, baculovirus-insect cell, mammalian)

Tailored buffer and formulation screens

Maleimide/iodoacetamide and NHS-mediated bioconjugation

Protein labeling with small molecules (dyes), peptides, oligonucleotides, and polymers (PEG)

Low-endotoxin preparation for in vivo studies

Custom purification schema for difficult proteins

Analytics

Rigorous biophysical and biochemical characterization to assess quality, stability, and developability. These analytical studies often follow functional evaluation in cell-based assays to confirm biologic activity and developability.

HPLC-based purity and aggregation profiling

Interferometry and plate-based stability assessment

Forced degradation studies

Antibody and protein developability assessments

In-use stability studies for formulation optimization

Comprehensive Biophysical & Analytical Toolkit

SPR & BLI

Real-time protein–protein binding kinetics

HPLC Systems

SEC, IEX, RP chromatography

Interferometry

Label-free stability and aggregation profiling

Plate Readers

UV-Vis absorbance and fluorescence

ELISA Platforms

Custom assay development and fit-for-purpose qualification for PK/PD studies

Mass Spectrometry

Molecular weight confirmation and PTM analysis

From Protein Production to Data-Driven Decisions

Protein expression and production with rigorous QC

Assay development and fit-for-purpose qualification

Comprehensive biophysical and analytical characterization

Analysis, interpretation, and reporting to support discovery decisions

These studies frequently inform downstream pharmacology that evaluate biologic activity in disease models.

Custom Assay Development for PK/PD Studies

Beyond standard analytics, Mosaic develops and validates custom ELISAs and immunoassays tailored to specific program needs, including de novo assay development for pharmacokinetic and pharmacodynamic testing across preclinical tissues.

Our flexible approach ensures quantitative, reproducible data suitable for discovery and regulatory support. These studies often support candidates emerging from antibody discovery and optimization programs.

Learn more about Mosaic’s integrated biologics discovery solutions supporting discovery, characterization, and biologic development.

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Start a conversation with our scientific team about your biologic discovery program.

Explore Other Discovery Solutions

Complementary capabilities supporting biologics discovery, engineering, and characterization.